ici 118551  (Tocris)

Journal: Brain, behavior, and immunity

Article Title: Social stress worsens colitis through β-adrenergic–driven oxidative stress in intestinal mucosal compartments

doi: 10.1016/j.bbi.2025.106222

Figure Lengend Snippet: Stress exacerbates C. rodentium -induced colitis severity, and β-adrenergic blockade modulates disease progression. (A) Timeline for C. rodentium -induced colitis + SDR paradigm. dPI = days post-infection. Mice were oral challenged with 3 × 10 7 CFU of C. rodentium at Day 4 of SDR paradigm (d0PI). (B-C) Body weight (BW) changes as % of baseline at (B) d3PI (after SDR) and (C) d9PI. (D) C. rodentium fecal load at d3PI, d7PI, and d9PI, after bacterial growth in MacConkey agar + kanamycin for 24 h. (E) LPS-binding protein (LBP) in serum. (F) Colon length; (G) Spleen weight to body weight (BW) ratio. (H) Total histopathology score of distal colon sections stained with H&E at d9PI. Higher scores indicate increased epithelial injury and mucosal inflammation; and representative H&E images of distal colon from undisturbed infected mice, stressed infected mice, and stressed infected mice treated with propranolol. Scale bars = 100 μm. (I) Quantification of DUOX2 fluorescence intensity and representative DUOX2 immunofluorescence images of distal colon sections from undisturbed infected mice, stressed infected mice, and stressed infected mice treated with propranolol. DUOX2 is shown in red and nuclei in blue (DAPI). Statistical analysis was performed by one-way ANOVA with post hoc comparisons: Control C. rodentium vs. Stress C. rodentium and Stress C. rodentium vs. Stress Propranolol C. rodentium . Unpaired t -test compared uninfected vs. infected undisturbed controls when applicable. Data are presented as mean ± SEM, with p < 0.05 considered statistically significant. n = 4–6/group.

Article Snippet: To selectively inhibit β2-adrenergic receptor (β2-AR) signaling, mice subjected to the SDR paradigm and C. rodentium infection were treated with zenidolol (ICI-118551) hydrochloride (cat. #S8114, Selleckchem, Houston, TX, USA).

Techniques: Biomarker Discovery, Infection, Binding Assay, Histopathology, Staining, Fluorescence, Immunofluorescence, Control

Journal: Brain, behavior, and immunity

Article Title: Social stress worsens colitis through β-adrenergic–driven oxidative stress in intestinal mucosal compartments

doi: 10.1016/j.bbi.2025.106222

Figure Lengend Snippet: Apocynin alleviates stress-induced exacerbation of C. rodentium - and DSS-induced colitis. (A-B) Timeline for C. rodentium - and DSS-induced colitis, respectively, combined with SDR paradigm. dPI = days post-infection. (C-E) Body weight (BW) change at (C) d2PI and (D) d9PI relative to infected unstressed mice, and at (E) D7 relative to water-treated unstressed mice. (G-H) Colon length as a percentage of (G) infected and (H) DSS-treated unstressed mice. (I-J) Fold change gene expression of colonic ROS/RNS-related enzymes ( Duox2, Duoxa2, Nos2 ) and chemokine Ccl2 , compared to (I) infected and (J) DSS-treated unstressed controls. Values were log-transformed prior to analysis and analyzed by t -test. (K-L) Quantification of DUOX2 fluorescence intensity and representative DUOX2 immunofluorescence images of distal colon sections from stressed infected mice and stressed infected mice treated with propranolol. DUOX2 is shown in red and nuclei in blue (DAPI). Data are presented as mean ± SEM; p < 0.05 was considered statistically significant. n = 4–6/group.

Article Snippet: To selectively inhibit β2-adrenergic receptor (β2-AR) signaling, mice subjected to the SDR paradigm and C. rodentium infection were treated with zenidolol (ICI-118551) hydrochloride (cat. #S8114, Selleckchem, Houston, TX, USA).

Techniques: Infection, Gene Expression, Transformation Assay, Fluorescence, Immunofluorescence